By using reduced sulfur to provide electron donors and energy sources, replacing the traditional method of adding carbon sources, Thiobacillus denitrificans in nitrogen-containing wastewater is significantly enriched and propagated. The competition among microbial populations is relatively low, and nitrate in the water body rapidly acquires electrons under the action of microorganisms, which are then reduced to nitrogen gas. The efficiency of this process is several times higher than that of traditional heterotrophic denitrification, and it is more economical.
